promocell cat Search Results


growth medium 2 supplement pack  (PromoCell)
99
PromoCell growth medium 2 supplement pack
Growth Medium 2 Supplement Pack, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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supplement pack  (PromoCell)
93
PromoCell supplement pack
Supplement Pack, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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supplement pack - by Bioz Stars, 2026-03
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skeletal muscle dm promocell  (PromoCell)
96
PromoCell skeletal muscle dm promocell
Skeletal Muscle Dm Promocell, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmvecs  (PromoCell)
86
PromoCell pmvecs
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Pmvecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mesenchymal stem cell growth medium 2  (PromoCell)
96
PromoCell mesenchymal stem cell growth medium 2
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Mesenchymal Stem Cell Growth Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mesenchymal stem cell growth medium 2 - by Bioz Stars, 2026-03
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ecgmmv2 medium  (PromoCell)
98
PromoCell ecgmmv2 medium
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Ecgmmv2 Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecgmmv2 medium - by Bioz Stars, 2026-03
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endothelial cell growth medium mv  (PromoCell)
97
PromoCell endothelial cell growth medium mv
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Endothelial Cell Growth Medium Mv, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cell growth medium mv - by Bioz Stars, 2026-03
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bronchial epithelial cell medium  (PromoCell)
95
PromoCell bronchial epithelial cell medium
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Bronchial Epithelial Cell Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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growth supplement  (PromoCell)
98
PromoCell growth supplement
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Growth Supplement, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human umbilical vein endothelial cells  (PromoCell)
99
PromoCell human umbilical vein endothelial cells
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Human Umbilical Vein Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonfluorescent human cardiac fibroblasts  (PromoCell)
97
PromoCell nonfluorescent human cardiac fibroblasts
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Nonfluorescent Human Cardiac Fibroblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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supplementmix  (PromoCell)
86
PromoCell supplementmix
CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed <t>PMVECs.</t> A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of <t>PMVECs</t> <t>transfected</t> with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.
Supplementmix, supplied by PromoCell, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed PMVECs. A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of PMVECs transfected with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Reduced carboxylesterase 1 is associated with endothelial injury in methamphetamine-induced pulmonary arterial hypertension

doi: 10.1152/ajplung.00453.2016

Figure Lengend Snippet: CES1 rs115629050 variant is associated with reduced enzymatic activity and increased apoptosis in unstimulated and METH-exposed PMVECs. A: CES1 activity assay of peripheral blood mononuclear cells from healthy donors (wild type) and METH-PAH patients carrying rs115629050. *P < 0.05, unpaired t-test. B: allelic discrimination assay for rs115629050 in gDNA samples extracted from lung tissue paraffin blocks (see methods). C: caspase 3/7 activity assay of PMVECs transfected with either GFP, wild-type (CES1), or rs115629050 mutant (mutant CES1) plasmids. **P < 0.01 and ***P < 0.001 vs. all other groups; ordinary 1-way ANOVA with Tukey’s multiple-comparison test.

Article Snippet: To achieve gene knockdown, 2 μM siRNA against CES1 (cat. no. L-009051-00GE; Dharmacon), CYP2D6 (siRNA ID no. s3834; Thermo Fisher), or nontargeting siRNA control (cat. no. D-001810-10-05GE; Dharmacon) was transfected into healthy PMVECs (cat. no. C-12282; Promocell).

Techniques: Variant Assay, Activity Assay, Transfection, Mutagenesis

CES1- and CYP2D6-deficient PMVECs have increased susceptibility to apoptosis. A: Western blot (WB)for CES1 in control (siCt) or CES siRNA-transfected healthy human PMVECs. B: caspase 3/7 activity assays of siCt and CES1 siRNA-transfected PMVECs at baseline and following METH (5 mM) exposure for 4 h. C and D: WB (C) and caspase 3/7 assay (D) for CYP2D6 in siCt or CYP2D6 (si2D6) siRNA-transfected healthy human PMVECs. Numbers under WB represent densitometry ratio of signal vs. tubulin from 3 independent studies. ***P < 0.001 vs. siCt + METH, ordinary 1-way ANOVA with Tukey’s multiple-comparison test.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Reduced carboxylesterase 1 is associated with endothelial injury in methamphetamine-induced pulmonary arterial hypertension

doi: 10.1152/ajplung.00453.2016

Figure Lengend Snippet: CES1- and CYP2D6-deficient PMVECs have increased susceptibility to apoptosis. A: Western blot (WB)for CES1 in control (siCt) or CES siRNA-transfected healthy human PMVECs. B: caspase 3/7 activity assays of siCt and CES1 siRNA-transfected PMVECs at baseline and following METH (5 mM) exposure for 4 h. C and D: WB (C) and caspase 3/7 assay (D) for CYP2D6 in siCt or CYP2D6 (si2D6) siRNA-transfected healthy human PMVECs. Numbers under WB represent densitometry ratio of signal vs. tubulin from 3 independent studies. ***P < 0.001 vs. siCt + METH, ordinary 1-way ANOVA with Tukey’s multiple-comparison test.

Article Snippet: To achieve gene knockdown, 2 μM siRNA against CES1 (cat. no. L-009051-00GE; Dharmacon), CYP2D6 (siRNA ID no. s3834; Thermo Fisher), or nontargeting siRNA control (cat. no. D-001810-10-05GE; Dharmacon) was transfected into healthy PMVECs (cat. no. C-12282; Promocell).

Techniques: Western Blot, Transfection, Activity Assay

CES1-deficient PMVECs demonstrate reduced autophagy in response to METH. A: nonexposed and 4-h METH exposed control (siCt) and CES1 (siCES1) siRNA-treated cells were fixed and stained with CellRox green. Fluorescence was measured relative to Ctrl-no METH. ***P < 0.001, unpaired t-test. B: WB of unstimulated and 4-h-METH control (Ctrl) and siCES1-treated cells for LC3. C: WB of bafilomycin-treated unstimulated and 4-h-METH treated Ctrl and siCES1 cells for LC3. Densitometry is measured relative to tubulin as a loading control. In B and C: *P < 0.05, **P < 0.01, and ***P < 0.001 vs. siCt no METH; ##P < 0.01, ###P < 0.001 vs. corresponding control; 1-way ANOVA with Bonferroni posttest (n = 3).

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Reduced carboxylesterase 1 is associated with endothelial injury in methamphetamine-induced pulmonary arterial hypertension

doi: 10.1152/ajplung.00453.2016

Figure Lengend Snippet: CES1-deficient PMVECs demonstrate reduced autophagy in response to METH. A: nonexposed and 4-h METH exposed control (siCt) and CES1 (siCES1) siRNA-treated cells were fixed and stained with CellRox green. Fluorescence was measured relative to Ctrl-no METH. ***P < 0.001, unpaired t-test. B: WB of unstimulated and 4-h-METH control (Ctrl) and siCES1-treated cells for LC3. C: WB of bafilomycin-treated unstimulated and 4-h-METH treated Ctrl and siCES1 cells for LC3. Densitometry is measured relative to tubulin as a loading control. In B and C: *P < 0.05, **P < 0.01, and ***P < 0.001 vs. siCt no METH; ##P < 0.01, ###P < 0.001 vs. corresponding control; 1-way ANOVA with Bonferroni posttest (n = 3).

Article Snippet: To achieve gene knockdown, 2 μM siRNA against CES1 (cat. no. L-009051-00GE; Dharmacon), CYP2D6 (siRNA ID no. s3834; Thermo Fisher), or nontargeting siRNA control (cat. no. D-001810-10-05GE; Dharmacon) was transfected into healthy PMVECs (cat. no. C-12282; Promocell).

Techniques: Staining, Fluorescence